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KMID : 0861020210360050081
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Korea Journal of Herbology
2021 Volume.36 No. 5 p.81 ~ p.91
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Effect of Lonicerae Japonicae Flos on Bone Densityin Ovariectomized Rat Model of Osteoporosis
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Lee Sung-Yub
Kim Min-Sun
Hong Soo-Yeon
Kim Jae-Hyun
Kim Hong-Sik
Lee Chung-Ho
Jung Hyuk-Sang
Sohn Young-Joo
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Abstract
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Objectives : Osteoporosis is a systemic skeletal disease that decreases bone density and increases the risk of fractures. Bisphosphonates and SERMs are mainly used to treat osteoporosis, but, long-term use increases the risk of side effects such as jaw bone necrosis and breast cancer. Therefore, it is necessary to develop a therapeutic agent for a natural product with few side effects. Water extract of Lonicerae Japonicae Flos (wLF) was mainly found to have anti-cancer and anti-inflammatory effects. However, the effect of wLF on osteoporosis has not been elucidated. Therefore, this experiment investigated the effect of wLF on osteoclasts, osteoblasts and osteoporosis models.
Methods : In order to study the effect of wLF on osteoporosis, the OVX-induced rat model was used for in vivo study. After 8 weeks, we measured body weight, uterine weight, liver weight, femur weight, bone density, trabecular area and tibia ash weight. To determine the effect of wLF on osteoclast differentiation, we measured the number of TRAP- positive cells and TRAP activity. To examine the effect of wLF on the expression of osteoblast-related genes, we measured the mRNA expression of alkaline phosphatase (ALP, Alpl) and osteocalcin (OCN, Bglap2).
Results : In vivo experiment, wLF inhibited the reduction of femur weight, trabecular area, bone density and tibia ash weight. In vitro experiment, wLF had no significant effect on osteoclast differentiation. However, wLF increased the mRNA expression of Alpl and Bglap2 in MC3T3-E1 cell.
Conclusions : This result suggested that wLF may be used for the treatment and prevention of postmenopausal osteoporosis.
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KEYWORD
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Water extract of Lonicerae Japonicae Flos, osteoporosis, osteoclast, osteoblast, ovariectomized
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